Dean called Bela a bitch (1), called Ruby a skank (1), slutty (1), and an "ugly broad" (1). He also asked Lilith if her plan was to become "queen bitch" (1).

Sam called Ruby a bitch once.

Ruby called Dean a "son of a bitch." (1)

And Bobby said his whole, "you're almost hell's bitch, so you can see hell's other bitches," thing.